We have developed a real-time quantitative PCR (qPCR) multiplex assay that identifies and quantifies minute amounts of mixed-species nuclear DNA of horse (Equus caballus), goat (Capra hircus)/sheep (Ovis aries), pig (Sus scrofa) and camel (Camelus dromedarius) in a species-specific manner. The assays ability to accurately detect and quantify target DNA in multi-species mixtures is crucial when the target specimen may be overwhelmed by the non-target specimen. We have evaluated the assay under different conditions so that it is applicable for investigating meat authentication and agri-food testing to ensure health and safety (this assay cannot be used for forensic purposes). The efficient determination of the species of origin will help reduce the potential risks that fraudulent/imitation and contaminated food products could pose to our food supply. Our assays ability to accurately identify and quantify species-specific DNA present in biological samples will also facilitate downstream analyses that generate DNA profiles of the specimen for genetic identity testing and traceability.
Pathogens Tested
Camelid
Equine
Ovine/Caprine
Porcine
Bovine
Method
Real-Time PCR.
Sample Type
Accredited:
Milk, Milk Powder, Tissue and EDTA Blood
Transport Condition
Samples should be transported at 4°C.
Turn Around Time (TAT)
TAT for routine samples is within 3 working days. Samples delivered before 11:00 AM will begin processing immediately resulting in shorter TAT. Urgent Samples must be delivered before 11:00 AM and will be charged double.
We have developed a real-time quantitative PCR (qPCR) multiplex assay that identifies and quantifies minute amounts of mixed-species nuclear DNA of horse (Equus caballus), goat (Capra hircus)/sheep (Ovis aries), pig (Sus scrofa) and camel (Camelus dromedarius) in a species-specific manner. The assays ability to accurately detect and quantify target DNA in multi-species mixtures is crucial when the target specimen may be overwhelmed by the non-target specimen. We have evaluated the assay under different conditions so that it is applicable for investigating meat authentication and agri-food testing to ensure health and safety (this assay cannot be used for forensic purposes). The efficient determination of the species of origin will help reduce the potential risks that fraudulent/imitation and contaminated food products could pose to our food supply. Our assays ability to accurately identify and quantify species-specific DNA present in biological samples will also facilitate downstream analyses that generate DNA profiles of the specimen for genetic identity testing and traceability.
Pathogens Tested
Camelid
Equine
Ovine/Caprine
Porcine
Bovine
Method
Real-Time PCR.
Sample Type
Accredited:
Milk, Milk Powder, Tissue and EDTA Blood
Transport Condition
Samples should be transported at 4°C.
Turn Around Time (TAT)
TAT for routine samples is within 3 working days. Samples delivered before 11:00 AM will begin processing immediately resulting in shorter TAT. Urgent Samples must be delivered before 11:00 AM and will be charged double.
