MBG Lab staffs are competent in various manual and automated extraction methodologies, in addition to this MBG Lab makes use of fully automated high end instruments for the extraction of nucleic acids.

• Manual spin column nucleic acid Extraction:
  Mainly used kits are Qiagen and Promega manual extraction kits.

• Automated Magnetic bead based extraction:
  MBG Lab houses Magna Pure (Roche), Nextractor (Genolution), Exiprep 96 lite (Bioneer), QIAsymphony (Qiagen) and Hamilton Chemagen (Hamilton), which are reliable robotic systems for rapid extraction of nucleic acids from different sample types.

Polymerase chain reaction (PCR) is a molecular biology technique that exponentially amplifies specific region of genome. The amplified PCR product can be detected for the presence or absence of micro-organisms, and it can be used also for other experiments including sequencing for mutation or fragment analysis.

• Conventional PCR & Gel Electrophoresis:
  

  The Lab houses high end thermal cyclers from Biorad, Applied biosystems and NYX technik both for 96 and 384 well reactions. Moreover, the lab is equipped with different systems of gel electrophoresis to capture and analyze DNA as well as RNA samples..

• Real-Time PCR:
  

  Real-Time PCR (qPCR) is used for detection and quantification of an amplified PCR product based on incorporation of a fluorescent reporter dye during amplification of DNA in real time. It is highly sensitive, accurate and efficient method of amplification and detection of DNA molecules.MBG Lab is equipped with light cycler instruments (Roche), Rotor-Gene Q (Qiagen) and ABI 7500 Fast Dx (Applied Biosystems), which can be used for mutation and pathogen detection, as well as gene detection, gene expression analysis, genetic variation analysis, and array data validation.

ABI Genetic Analyzer is an automated capillary based genetic analyzer designed for a wide range of applications, such as sequencing, linkage analysis, STR analysis, SNP detection, discovery and validation, mutation detection, and other applications.

MBG Lab has two different array analyzers:

• Open source Microarray:
  

  MBG Lab has the Q Array2 microarray printing machine (Genetix) and a GenePix scanner (Axon Instruments) which are open source and used for in-house SNP and expression microarray printing and scanning.

• Commercial Microarray:
  

  MBG Lab houses the state-of-the art, high resolution Gene Titan Multi-Channel (MC). The instrument is an automated High-throughput solution for monitoring gene expression and genome-wide SNP genotyping.

Next Generation Sequencing (NGS) technology allows us to sequence the whole genome faster and at a lower cost than the previously used Sanger sequencing, and as such has revolutionized the study of genomics and molecular biology.
MBG Lab houses two Illumina Next Generation Sequencers, the MiSeq and the NextSeq500, enabling the Lab to expand its repertoire of services into areas such as meta-genomics, whole genome &exome sequencing along with high throughput targeted panel sequencing..

MBG Lab is equipped with the latest molecular cytogenetic equipments. Data is analysed using BX62 Fluorescent Microscope (Olympus) with Genus Software for image analysis (applied Imaging). This technology is used for different applications, such as:

• Chromosomal Analysis (Karyptyping)

• Fluorescent in situ hybridization (FISH)

MBG Lab has the picking and sub-clone management system, QPIX2, (Genetix). This machine enables tasks such as picking colonies (e.g. E. coli, yeast, phage, etc), replicating plates/libraries, and setting up screens as well as cherry picking wells of interest. Further applications include rearraying (hit picking), Y2H, SGA, gridding and phage display, Copying and screening BAC libraries, membrane array genomic work, construction of libraries etc.

• MBG STEM:
  

  Stem cell therapy is a Regenerative Veterinary Cell Therapy that delivers millions of regenerative cells from a small sample of an animal’s adipose tissue (fat), for the treatment of a variety of conditions, some of which are:

  • Joints (OCD, Arthritis)

  • Tendons

  • Ligaments

  • Fractures

• PLATELET-RICH-PLASMA (PRP):
  

  This is an exceptional way to treat joints injuries and interestingly it can be administered to the injured animal in conjunction with Stem cell therapy for a better outcome. Presently, MBG Lab is offering an on-site processing service for PRP.

MBG Lab Houses, the cobas e 601 module , a fully automated analyzer that uses a patented electrochemiluminescence (ECL) technology for immunoassay analysis. It is designed for both quantitative and qualitative in vitro assay determinations for a broad range of applications (including anemia; bone, cardiac and tumor markers; critical care; fertility/hormones; maternal care; and infectious diseases).

Electrochemiluminescence is a technology for immunoassay detection. The development of ECL immunoassays is based on the use of a ruthenium-complex and tripropylamine (TPA). The chemiluminescence reaction for the detection of the reaction complex is initiated by applying a voltage to the sample solution resulting in a precisely controlled reaction. ECL technology can accommodate many immunoassay principles while providing superior performance.

MBG Lab has IT infrastructure consists of all elements that support the management and usability of data and information. These include the physical hardware and facilities (off-site data center), data storage and retrieval, highly secure network systems, and validated in-house software to support the business goals.

MBG Lab Bioinformatics Facility offers Next-Generation Sequencing (NGS) analyses using state-of-the-art methods which rely on a highly scalable NGS analysis system developed in-house using high-performance computing (HPC) infrastructure, databases and other relevant resources. It currently comprises analysis pipelines for whole-genome sequencing (WGS), whole-exome sequencing (WES), cancer panel sequencing and metagenomics.