Being one of the best-equipped genetic laboratories in the country, we are proud to offer technical and hands-on courses for various molecular biology applications.
MBG Lab provides a full spectrum of research training opportunities with the underlying common theme of Molecular Genetics.
These courses are given in a long or short form depending on the expertise of the students.
The long course will include more theoretical background in addition to the practical course, while the short course covers mostly practical hands-on training.
Irrespective of the course duration all trainees who have completed the training will receive a certificate of attendance.
We are happy to customize a training for a group to meet your requirement.
For further information, please do not hesitate to contact us.
Please, note that training are available only during a specific periods of the year.
Modules:
Module :
IET01
Module Name :
Nucleic Acid Extraction
Duration :
5 days
Pre-requisition :
Description :
Extraction is typically the first step in a longer laboratory process. it is an important part of that process as the nucleic acid first needs to be purified away from proteins and other cellular contaminant.
This course includes training for manual and automated DNA extraction methods. Automated DNA extraction technologies include filter based and magnetic bead based extraction methods, using different instruments.
Module :
IET02A
Module Name :
PCR, RT-PCR & Gel Electrophoresis
Duration :
5 days
Pre-requisition :
IET01
Description :
PCR is the key technique in molecular biology. It is a simple and inexpensive tool to amplify a segment of DNA. It is
widely used in research and molecular diagnostics.
The 5 day PCR training course aims to provide step by step technical assistance in various aspects of PCR setup and analysis. This includes Reverse transcription(RT)/cDNA synthesis,End-point PCR setup and analysis by agarose gel electrophoresis, Real Time PCR,SYBR Green, Hybridization probeand Hydrolysis probe based Real Time PCR.
The course includes training for both conventional PCR and Real time PCR as well as gel electrophoresis techniques. Various methods of DNA amplifications and detections are used in the training.
Module :
IET02B
Module Name :
Real-Time PCR
Duration :
5 days
Pre-requisition :
IET01 & IET02A
Description :
PCR is the key technique in molecular biology. It is a simple and inexpensive tool to amplify a segment of DNA. It is
widely used in research and molecular diagnostics.
The 5 day PCR training course aims to provide step by step technical assistance in various aspects of PCR setup and analysis. This includes Reverse transcription(RT)/cDNA synthesis,End-point PCR setup and analysis by agarose gel electrophoresis, Real Time PCR,SYBR Green, Hybridization probeand Hydrolysis probe based Real Time PCR.
The course includes training for both conventional PCR and Real time PCR as well as gel electrophoresis techniques. Various methods of DNA amplifications and detections are used in the training.
Module :
IET03
Module Name :
Sequencing
Duration :
5 days
Pre-requisition :
IET01, IET02A & IET02B
Description :
Sequencing is the process of reading the nucleotide bases in a DNA molecule.
Knowledge of DNA sequences has become in dispensible for basic biological research and in numerous applied fields such as diagnostics, biotechnology,forensic biology and biological systematics.
This course gives training on sequencing using ABI 3730, and 3730xl (Applied Biosystems). The course offers students a hands-on experience in sequencing, from the cycle sequencing step to sequence analysis and submitting the sequence on GenBank database. The course also incorporates an introduction to basic Bioinformatics which includes using NCBI, BLAST tools,repeat finder softwares to name a few ..
Module :
IET04
Module Name :
Genotyping
Duration :
5 days
Pre-requisition :
IET01, IET02A & IET02B
Description :
Genotyping is the process of determining differences in the genetic make-up of an individual by examining the Individual's DNA using bioassays and comparing it to another individual's or reference sequence.
The training course is aimed at providing the basic molecular skills, in particular, fluorescent microsatellite genotyping. This includes hands-on training using state-of-the-art equipment as well as different analysis and reporting software.
Module :
IET05A
Module Name :
Bioinformatics : R package (Beginner)
Duration :
5 days
Pre-requisition :
Basic Knowledge in computer manipulation and statistics.
Description :
R is an integrated suite of software facilities for data manipulation, calculation and graphical display. The course will cover the following section:
Data handling and storage facility;
Use a suite of operators for calculations on arrays, in particular matrices;
Use a large, coherent, integrated collection of intermediate tools for data analysis;
Introduction to the graphical facilities for data analysis and display either directly at the computer or on hardcopy;
Develope, simple and effective programming language which includes conditionals, loops, user defined recursive functions and input and output facilities.
Module :
IET05B
Module Name :
Bioinformatics : R package (Advanced)
Duration :
5 days
Pre-requisition :
Basic Knowledge in computer manipulation and statistics and the introductory module IET05A
Description :
R is an integrated suite of software facilities for data manipulation, calculation and graphical display. All R functions and datasets are stored in packages. The package Bioconductor provides tools for the analysis and comprehension of high-throughput genomic data.
The course will cover the following section:
Introduction to Bioconductor;
Microarray data analysis using R/Bioconductor;
RNA-seq data analysis and differential expression using R/Bioconductor;
Data Clustering and classification using R.
Module :
IET05C
Module Name :
Galaxy Tools
Duration :
5 days
Pre-requisition :
Basic Knowledge in statistics and genomics.
Description :
The Galaxy workflow provides a set of tools for the manipulation and analysis of genomic data. It is very intuitive in use making it a tool of choice for the biologist. Indeed, Galaxy is a platform that offers a "constellation" of tools to analyze, manipulate and visualize genomic data, without the need for programming knowledge.
The course will cover the following section:
data operations: sum, average, subtract, calculate the coverage of a given region;
Sequence analysis: calculate correlations, use EMBOSS tools, align sequencing data;
visualization of the data: display multiple alignments, generate graphs;
Complete ChIP-Seq data analysis workflow.
Module :
IET06A
Module Name :
Cytogenetics
Duration :
5 days
Pre-requisition :
Description :
Cytogenetics encompasses all aspects of chromosome biology applied in all areas of biomedicine. These include the structural and functional organization of the chromosomes and the nucleus, genome variation, chromosomal abnormalities, etc ...
During the 3 days (split over the weekend) training course, students will perform various cytogenetic techniques such as harvesting and preparing slides from blood cultures, specialized stainings like G & C banding and silver staining.
The 3 day FISH training course includes preparing direct or indirect fluorescent probes using Nick translation or random priming as well as using these probes to perform FISH.
The prepared slides will then be scanned and analyzed using computer-imaging systems.
Module :
IET06B
Module Name :
FISH
Duration :
5 days
Pre-requisition :
IET06A
Description :
Module :
IET07
Module Name :
Microarray
Duration :
5 days
Pre-requisition :
IET01
Description :
Microarray technology is a powerful technique that helps to investigate thousands of genes for their expressions or
mutation simultaneously.
A 5-day microarray hands-on training and background courses will be offered to students at MBG lab. This includes experimental design and the use of different data mining tools(software). The course will cover technical presentation, printing microarray slides, hybridization, washing of the slides,scanning and analysis.
Education and Training
Being one of the best-equipped genetic laboratories in the country, we are proud to offer technical and hands-on courses for various molecular biology applications.
MBG Lab provides a full spectrum of research training opportunities with the underlying common theme of Molecular Genetics.
These courses are given in a long or short form depending on the expertise of the students.
The long course will include more theoretical background in addition to the practical course, while the short course covers mostly practical hands-on training.
Irrespective of the course duration all trainees who have completed the training will receive a certificate of attendance.
We are happy to customize a training for a group to meet your requirement.
For further information, please do not hesitate to contact us.
Please, note that training are available only during a specific periods of the year.
Modules:
Module :
IET01
Module Name :
Nucleic Acid Extraction
Duration :
5 days
Pre-requisition :
Description :
Extraction is typically the first step in a longer laboratory process. it is an important part of that process as the nucleic acid first needs to be purified away from proteins and other cellular contaminant.
This course includes training for manual and automated DNA extraction methods. Automated DNA extraction technologies include filter based and magnetic bead based extraction methods, using different instruments.
Module :
IET02A
Module Name :
PCR, RT-PCR & Gel Electrophoresis
Duration :
5 days
Pre-requisition :
IET01
Description :
PCR is the key technique in molecular biology. It is a simple and inexpensive tool to amplify a segment of DNA. It is
widely used in research and molecular diagnostics.
The 5 day PCR training course aims to provide step by step technical assistance in various aspects of PCR setup and analysis. This includes Reverse transcription(RT)/cDNA synthesis,End-point PCR setup and analysis by agarose gel electrophoresis, Real Time PCR,SYBR Green, Hybridization probeand Hydrolysis probe based Real Time PCR.
The course includes training for both conventional PCR and Real time PCR as well as gel electrophoresis techniques. Various methods of DNA amplifications and detections are used in the training.
Module :
IET02B
Module Name :
Real-Time PCR
Duration :
5 days
Pre-requisition :
IET01 & IET02A
Description :
PCR is the key technique in molecular biology. It is a simple and inexpensive tool to amplify a segment of DNA. It is
widely used in research and molecular diagnostics.
The 5 day PCR training course aims to provide step by step technical assistance in various aspects of PCR setup and analysis. This includes Reverse transcription(RT)/cDNA synthesis,End-point PCR setup and analysis by agarose gel electrophoresis, Real Time PCR,SYBR Green, Hybridization probeand Hydrolysis probe based Real Time PCR.
The course includes training for both conventional PCR and Real time PCR as well as gel electrophoresis techniques. Various methods of DNA amplifications and detections are used in the training.
Module :
IET03
Module Name :
Sequencing
Duration :
5 days
Pre-requisition :
IET01, IET02A & IET02B
Description :
Sequencing is the process of reading the nucleotide bases in a DNA molecule.
Knowledge of DNA sequences has become in dispensible for basic biological research and in numerous applied fields such as diagnostics, biotechnology,forensic biology and biological systematics.
This course gives training on sequencing using ABI 3730, and 3730xl (Applied Biosystems). The course offers students a hands-on experience in sequencing, from the cycle sequencing step to sequence analysis and submitting the sequence on GenBank database. The course also incorporates an introduction to basic Bioinformatics which includes using NCBI, BLAST tools,repeat finder softwares to name a few ..
Module :
IET04
Module Name :
Genotyping
Duration :
5 days
Pre-requisition :
IET01, IET02A & IET02B
Description :
Genotyping is the process of determining differences in the genetic make-up of an individual by examining the Individual's DNA using bioassays and comparing it to another individual's or reference sequence.
The training course is aimed at providing the basic molecular skills, in particular, fluorescent microsatellite genotyping. This includes hands-on training using state-of-the-art equipment as well as different analysis and reporting software.
Module :
IET05A
Module Name :
Bioinformatics : R package (Beginner)
Duration :
5 days
Pre-requisition :
Basic Knowledge in computer manipulation and statistics.
Description :
R is an integrated suite of software facilities for data manipulation, calculation and graphical display. The course will cover the following section:
Data handling and storage facility;
Use a suite of operators for calculations on arrays, in particular matrices;
Use a large, coherent, integrated collection of intermediate tools for data analysis;
Introduction to the graphical facilities for data analysis and display either directly at the computer or on hardcopy;
Develope, simple and effective programming language which includes conditionals, loops, user defined recursive functions and input and output facilities.
Module :
IET05B
Module Name :
Bioinformatics : R package (Advanced)
Duration :
5 days
Pre-requisition :
Basic Knowledge in computer manipulation and statistics and the introductory module IET05A
Description :
R is an integrated suite of software facilities for data manipulation, calculation and graphical display. All R functions and datasets are stored in packages. The package Bioconductor provides tools for the analysis and comprehension of high-throughput genomic data.
The course will cover the following section:
Introduction to Bioconductor;
Microarray data analysis using R/Bioconductor;
RNA-seq data analysis and differential expression using R/Bioconductor;
Data Clustering and classification using R.
Module :
IET05C
Module Name :
Galaxy Tools
Duration :
5 days
Pre-requisition :
Basic Knowledge in statistics and genomics.
Description :
The Galaxy workflow provides a set of tools for the manipulation and analysis of genomic data. It is very intuitive in use making it a tool of choice for the biologist. Indeed, Galaxy is a platform that offers a "constellation" of tools to analyze, manipulate and visualize genomic data, without the need for programming knowledge.
The course will cover the following section:
data operations: sum, average, subtract, calculate the coverage of a given region;
Sequence analysis: calculate correlations, use EMBOSS tools, align sequencing data;
visualization of the data: display multiple alignments, generate graphs;
Complete ChIP-Seq data analysis workflow.
Module :
IET06A
Module Name :
Cytogenetics
Duration :
5 days
Pre-requisition :
Description :
Cytogenetics encompasses all aspects of chromosome biology applied in all areas of biomedicine. These include the structural and functional organization of the chromosomes and the nucleus, genome variation, chromosomal abnormalities, etc ...
During the 3 days (split over the weekend) training course, students will perform various cytogenetic techniques such as harvesting and preparing slides from blood cultures, specialized stainings like G & C banding and silver staining.
The 3 day FISH training course includes preparing direct or indirect fluorescent probes using Nick translation or random priming as well as using these probes to perform FISH.
The prepared slides will then be scanned and analyzed using computer-imaging systems.
Module :
IET06B
Module Name :
FISH
Duration :
5 days
Pre-requisition :
IET06A
Description :
Module :
IET07
Module Name :
Microarray
Duration :
5 days
Pre-requisition :
IET01
Description :
Microarray technology is a powerful technique that helps to investigate thousands of genes for their expressions or
mutation simultaneously.
A 5-day microarray hands-on training and background courses will be offered to students at MBG lab. This includes experimental design and the use of different data mining tools(software). The course will cover technical presentation, printing microarray slides, hybridization, washing of the slides,scanning and analysis.
