Molecular based methods like PCR, Real-Time PCR and more recently next generation sequencing (NGS) have revolutionized the field of veterinary diagnostics.
At MBG, we offer detection of pathogenic viruses, bacteria and parasites using molecular methods, which are fast and highly sensitive to detect microbial
pathogens in various specimens.
MBG is an ISO 17025 accredited facility and benefits from an isolated containment level 3 facility for processing highly (level 3) contagious pathogens.
Newcastle disease virus (NDV) is a single-stranded RNA virus of the Paramyxoviridae family that causes a contagious disease in birds. The linear RNA genome comprises just over 15K nucleotides that encode 6 genes. The spherical viral particle is enveloped and has external spikes formed by two proteins, hemagglutinin and neuraminidase.
NDV has been shown to be able to infect over 200 species of birds, but the severity of disease produced varies with both host and strain of virus. The virus can survive for several weeks in warm environments such as in manure or between bird feathers and enters the host by ingestion of contaminated food stuff or contact with contaminated bodily fluids.
NDV strains can be categorised as velogenic (highly virulent), mesogenic (intermediate virulence), or lentogenic (nonvirulent). Velogenic strains produce severe nervous and respiratory signs, spread rapidly, and cause up to 90% mortality. Mesogenic strains cause coughing, affect egg quality and production, and result in up to 10% mortality. Lentogenic strains produce mild signs with negligible mortality.
Method
Real-Time RT-PCR.
Sample Type
Swab/Secretion (Respiratory), Swab/Secretion (Rectal), Tissue,Tissue on FTA card, Culture.
Transport Condition
Samples should be transported at 4°C. Stool must be frozen after collection and delivered within 24 hours.
Specimens must be sent in RNA preservative media (except culture).
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 3 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Nipah virus is an enveloped, negative-sense, single-stranded RNA virus in the family Paramyxoviridae,
genus Henipavirus. Primary reservoir for Nipah virus are fruit bats of the genus Pteropus. Domestic swine are extremely susceptible to infection and act as amplifying host. However, infections have also been reported in dogs, cats, horses and goats. Swine infected with Nipah virus may cause aerosols or transmit the disease by direct contact of their respiratory
secretions to other swine. Incubation period in pigs is approximately 7–14 days, but may be as short as four days. Intranasal and oral inoculation of cats with virus experimentally produced disease and incubation periods of 6–8 days have been documented.
Method
Real Time RT-PCR
Sample Type
EDTA Blood, CSF, Swab / Secretion (Respiratory).
Transport Condition
Samples should be transported at 4°C. Urine sample must be frozen after collection and delivered within 24 hours. Specimens must be sent in RNA Preservative media.
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 3 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Molecular based methods like PCR, Real-Time PCR and more recently next generation sequencing (NGS) have revolutionized the field of veterinary diagnostics.
At MBG, we offer detection of pathogenic viruses, bacteria and parasites using molecular methods, which are fast and highly sensitive to detect microbial
pathogens in various specimens.
MBG is an ISO 17025 accredited facility and benefits from an isolated containment level 3 facility for processing highly (level 3) contagious pathogens.
Newcastle disease virus (NDV) is a single-stranded RNA virus of the Paramyxoviridae family that causes a contagious disease in birds. The linear RNA genome comprises just over 15K nucleotides that encode 6 genes. The spherical viral particle is enveloped and has external spikes formed by two proteins, hemagglutinin and neuraminidase.
NDV has been shown to be able to infect over 200 species of birds, but the severity of disease produced varies with both host and strain of virus. The virus can survive for several weeks in warm environments such as in manure or between bird feathers and enters the host by ingestion of contaminated food stuff or contact with contaminated bodily fluids.
NDV strains can be categorised as velogenic (highly virulent), mesogenic (intermediate virulence), or lentogenic (nonvirulent). Velogenic strains produce severe nervous and respiratory signs, spread rapidly, and cause up to 90% mortality. Mesogenic strains cause coughing, affect egg quality and production, and result in up to 10% mortality. Lentogenic strains produce mild signs with negligible mortality.
Method
Real-Time RT-PCR.
Sample Type
Swab/Secretion (Respiratory), Swab/Secretion (Rectal), Tissue,Tissue on FTA card, Culture.
Transport Condition
Samples should be transported at 4°C. Stool must be frozen after collection and delivered within 24 hours.
Specimens must be sent in RNA preservative media (except culture).
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 3 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Nipah virus is an enveloped, negative-sense, single-stranded RNA virus in the family Paramyxoviridae,
genus Henipavirus. Primary reservoir for Nipah virus are fruit bats of the genus Pteropus. Domestic swine are extremely susceptible to infection and act as amplifying host. However, infections have also been reported in dogs, cats, horses and goats. Swine infected with Nipah virus may cause aerosols or transmit the disease by direct contact of their respiratory
secretions to other swine. Incubation period in pigs is approximately 7–14 days, but may be as short as four days. Intranasal and oral inoculation of cats with virus experimentally produced disease and incubation periods of 6–8 days have been documented.
Method
Real Time RT-PCR
Sample Type
EDTA Blood, CSF, Swab / Secretion (Respiratory).
Transport Condition
Samples should be transported at 4°C. Urine sample must be frozen after collection and delivered within 24 hours. Specimens must be sent in RNA Preservative media.
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 3 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
