Molecular Biology & Genomics Centre

Pathogen Identification


Molecular based methods like PCR, Real-Time PCR and more recently next generation sequencing (NGS) have revolutionized the field of veterinary diagnostics. At MBG, we offer detection of pathogenic viruses, bacteria and parasites using molecular methods, which are fast and highly sensitive to detect microbial pathogens in various specimens. MBG is an ISO 17025 accredited facility and benefits from an isolated containment level 3 facility for processing highly (level 3) contagious pathogens.


All Bacteria Virus Fungi Parasite


Accredited
Assay Code APN-020
Description In September 2012, health authorities were notified of several cases of severe hCoV infection caused by a novel virus type hCoV-EMC. The strain was redefined by the International Committee on Taxonomy of Viruses into Middle East respiratory syndrome coronavirus (MERS-CoV) since it was first reported in Saudi Arabia.

MERS-CoV is a beta coronavirus and causes respiratory infection of humans and dromedary camels. Several studies have confirmed that Dromedary camels (Camelus dromedarius) are the natural host and zoonotic source of the MERS-CoV infection in humans. Other animal species may also be susceptible to infection with MERS-CoV. However, their epidemiological significance has not been proven.

Positive RT-PCR results for MERS-CoV or isolation of the virus from dromedary camels are notifiable to the OIE . While the impact of MERS-CoV on animal health is very low, human infections have a significant public health impact.

Method Real-Time RT-PCR.
Sample Type
Accredited : Swab / Secretion (Respiratory).
Alternatives : Culture, Serum.
Transport Condition Samples should be transported at 4°C.
Specimens must be sent in RNA preservative media.
Turn Around Time (TAT) Normal Turnaround time for pathogen Identification is within 3 working days.
Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.

Links

Validated
Assay Code See Below
Description Monkeypox is a viral zoonotic disease, caused by Monkeypox virus which is recognized as the most important orthopoxvirus infecton after the eradication of smallpox.
Monkeypox virus belongs to the Orthopoxvirus genus in the family Poxviridae. The Orthopoxvirus genus also includes variola virus (which causes smallpox), vaccinia virus (used in the smallpox vaccine), and cowpox virus.
Monkeypox was first discovered in 1958 when two outbreaks of a pox-like disease occurred in colonies of monkeys kept for research, hence the name Monkeypox. The first human case of Monkeypox was recorded in 1970 in the Democratic Republic of the Congo (DRC) during a period of intensified effort to eliminate smallpox
Monkeypox is an infectious viral disease that can occur in both humans and some other animals.

Method APM-215: Orthopox virus detection & confirmation by PCR & Sequencing.
APM-216: Monkeypox virus detection by Real-Time PCR.


Sample Type
Tissue (Skin lesions), Swabs/Secretions(Abscess/lesions)
Transport Condition Samples should be transported at 4°C and delivered within 24 hours.
Turn Around Time (TAT) Normal Turnaround time for pathogen Identification is within 3 working days.
Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.

Links




Molecular Biology & Genomics Centre

Pathogen Identification


Molecular based methods like PCR, Real-Time PCR and more recently next generation sequencing (NGS) have revolutionized the field of veterinary diagnostics. At MBG, we offer detection of pathogenic viruses, bacteria and parasites using molecular methods, which are fast and highly sensitive to detect microbial pathogens in various specimens. MBG is an ISO 17025 accredited facility and benefits from an isolated containment level 3 facility for processing highly (level 3) contagious pathogens.


All Bacteria Virus Fungi Parasite


Accredited
Assay Code APN-020
Description In September 2012, health authorities were notified of several cases of severe hCoV infection caused by a novel virus type hCoV-EMC. The strain was redefined by the International Committee on Taxonomy of Viruses into Middle East respiratory syndrome coronavirus (MERS-CoV) since it was first reported in Saudi Arabia.

MERS-CoV is a beta coronavirus and causes respiratory infection of humans and dromedary camels. Several studies have confirmed that Dromedary camels (Camelus dromedarius) are the natural host and zoonotic source of the MERS-CoV infection in humans. Other animal species may also be susceptible to infection with MERS-CoV. However, their epidemiological significance has not been proven.

Positive RT-PCR results for MERS-CoV or isolation of the virus from dromedary camels are notifiable to the OIE . While the impact of MERS-CoV on animal health is very low, human infections have a significant public health impact.

Method Real-Time RT-PCR.
Sample Type
Accredited : Swab / Secretion (Respiratory).
Alternatives : Culture, Serum.
Transport Condition Samples should be transported at 4°C.
Specimens must be sent in RNA preservative media.
Turn Around Time (TAT) Normal Turnaround time for pathogen Identification is within 3 working days.
Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.

Links

Validated
Assay Code See Below
Description Monkeypox is a viral zoonotic disease, caused by Monkeypox virus which is recognized as the most important orthopoxvirus infecton after the eradication of smallpox.
Monkeypox virus belongs to the Orthopoxvirus genus in the family Poxviridae. The Orthopoxvirus genus also includes variola virus (which causes smallpox), vaccinia virus (used in the smallpox vaccine), and cowpox virus.
Monkeypox was first discovered in 1958 when two outbreaks of a pox-like disease occurred in colonies of monkeys kept for research, hence the name Monkeypox. The first human case of Monkeypox was recorded in 1970 in the Democratic Republic of the Congo (DRC) during a period of intensified effort to eliminate smallpox
Monkeypox is an infectious viral disease that can occur in both humans and some other animals.

Method APM-215: Orthopox virus detection & confirmation by PCR & Sequencing.
APM-216: Monkeypox virus detection by Real-Time PCR.


Sample Type
Tissue (Skin lesions), Swabs/Secretions(Abscess/lesions)
Transport Condition Samples should be transported at 4°C and delivered within 24 hours.
Turn Around Time (TAT) Normal Turnaround time for pathogen Identification is within 3 working days.
Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.

Links