Molecular Biology & Genomics Centre

Pathogen Identification


Molecular based methods like PCR, Real-Time PCR and more recently next generation sequencing (NGS) have revolutionized the field of veterinary diagnostics. At MBG, we offer detection of pathogenic viruses, bacteria and parasites using molecular methods, which are fast and highly sensitive to detect microbial pathogens in various specimens. MBG is an ISO 17025 accredited facility and benefits from an isolated containment level 3 facility for processing highly (level 3) contagious pathogens.


All Bacteria Virus Fungi Parasite


Validated
Assay Code APF-264
Description Feline Immunodeficiency Virus (FIV) is a retrovirus that affects cats worldwide. It weakens a cat's immune system over time, leaving them more susceptible to infections and certain cancers. FIV is primarily transmitted through bite wounds, so outdoor cats and those with a history of fighting are at higher risk. It's important for cat owners to be aware of FIV and take preventive measures, such as keeping cats indoors and getting them tested regularly.
Symptoms of cat FIV include poor coat condition, fever that keeps coming back, lack of appetite, inflammation in the mouth and gums,chronic or recurrent infections in the eyes, skin, upper respiratory tract, or bladder, constant diarrhea, persistent eye problems and seizures.
Two sets of primers were designed at a universal thermal cycling condition, and evaluated for detection of FIV RNA by real-time RT-PCR, which will be useful in early diagnosis and consequently addressing the threat of FIV agents in cats.

Method Real Time RT-PCR
Sample Type
EDTA Blood
Transport Condition Sample should be transported at 4°C.
Turn Around Time (TAT) Normal Turnaround time for pathogen Identification is within 3 working days.
Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.

Links




Molecular Biology & Genomics Centre

Pathogen Identification


Molecular based methods like PCR, Real-Time PCR and more recently next generation sequencing (NGS) have revolutionized the field of veterinary diagnostics. At MBG, we offer detection of pathogenic viruses, bacteria and parasites using molecular methods, which are fast and highly sensitive to detect microbial pathogens in various specimens. MBG is an ISO 17025 accredited facility and benefits from an isolated containment level 3 facility for processing highly (level 3) contagious pathogens.


All Bacteria Virus Fungi Parasite


Validated
Assay Code APF-264
Description Feline Immunodeficiency Virus (FIV) is a retrovirus that affects cats worldwide. It weakens a cat's immune system over time, leaving them more susceptible to infections and certain cancers. FIV is primarily transmitted through bite wounds, so outdoor cats and those with a history of fighting are at higher risk. It's important for cat owners to be aware of FIV and take preventive measures, such as keeping cats indoors and getting them tested regularly.
Symptoms of cat FIV include poor coat condition, fever that keeps coming back, lack of appetite, inflammation in the mouth and gums,chronic or recurrent infections in the eyes, skin, upper respiratory tract, or bladder, constant diarrhea, persistent eye problems and seizures.
Two sets of primers were designed at a universal thermal cycling condition, and evaluated for detection of FIV RNA by real-time RT-PCR, which will be useful in early diagnosis and consequently addressing the threat of FIV agents in cats.

Method Real Time RT-PCR
Sample Type
EDTA Blood
Transport Condition Sample should be transported at 4°C.
Turn Around Time (TAT) Normal Turnaround time for pathogen Identification is within 3 working days.
Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.

Links