Molecular Biology & Genomics Centre

Pathogen Identification


Molecular based methods like PCR, Real-Time PCR and more recently next generation sequencing (NGS) have revolutionized the field of veterinary diagnostics. At MBG, we offer detection of pathogenic viruses, bacteria and parasites using molecular methods, which are fast and highly sensitive to detect microbial pathogens in various specimens. MBG is an ISO 17025 accredited facility and benefits from an isolated containment level 3 facility for processing highly (level 3) contagious pathogens.


All Bacteria Virus Fungi Parasite


Accredited *
Assay Code See Below
Description African horse sickness (AHS) virus is a double stranded RNA virus of the family Reoviridae and causes African horse sickness in horses and related species such as mules, donkeys, and zebras. Horses are most severely affected by the disease. AHS is endemic in the central tropical regions of Africa and has occasionally extended to Egypt, the Middle East and the southern Arabian Peninsula.

The disease spreads mainly by the bite of insects such as midges (Culicoides) but direct horse to horse transmission does not occur. The virus can only survive through continuous cycles of transmission between its hosts-horses and insects. It does not survive in the environment outside of the host. Clinical signs are typically seen five to seven days after infection in the form of fever, redness of the inside surface of the eyelids. The disease progresses to one of the following forms: pulmonary form, cardiac form or mixed form.

There are nine distinct serotypes of AHSV (AHSV-1 to AHSV-9) . Any of the strains can cause disease with severity ranging from a mild fever to sudden death. The serotypes can be distinguished in serum neutralisation tests by the specificity of their reactions with neutralising antibodies. Molecular methods for determination of AHSV serotypes have been developed based on a set of nine individual ‘typing’ assays to detect and identify Seg-2 of each AHSV serotype.

Pathogens Tested
  • APA-001 : African horse sickness virus*
  • APA-182 : African horse sickness virus Serotyping

* Accredited Tests.

Method Real -Time RT-PCR
Sample Type
Accredited : EDTA Blood, Tissue (Spleen, Lung, Lymph nodes) , Culture.
Transport Condition Samples should be transported at 4°C and delivered within 24h of collection
Turn Around Time (TAT) Normal Turnaround time for pathogen Identification is within 3 working days.
Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.

Links

Validated
Assay Code APA-002
Description The adenoviruses are a group of non-enveloped, icosahedral DNA viruses. Adenovirus infections are ubiquitous in commercially farmed birds, and probably in all avian species.

The avian adenoviruses can be divided into three groups. Group I, or conventional adenoviruses, share a common group antigen. Group II adenoviruses include the viruses of turkey haemorrhagic enteritis (THE), marble spleen disease (MSD) and group II splenomegaly of chickens. These viruses also share a common antigen. Group III viruses, the egg drop syndrome (EDS) viruses, are widely distributed in waterfowl but can easily infect chickens, resulting in the production of abnormal eggshells. While many infections are subclinical and appear to be of little economic or welfare importance, significant outbreaks of disease associated with adenovirus do occur.

Method PCR and gel electrophoresis
Sample Type
Stool, Tissue (trachea, bursa, nasal mucosa, pharynx, lungs, kidney), Culture, Swab/Secretion (Rectal)
Transport Condition Samples should be transported at 4°C and delivered within 24h of collection.
Turn Around Time (TAT) Normal Turnaround time for pathogen Identification is within 3 working days.
Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.

Links




Molecular Biology & Genomics Centre

Pathogen Identification


Molecular based methods like PCR, Real-Time PCR and more recently next generation sequencing (NGS) have revolutionized the field of veterinary diagnostics. At MBG, we offer detection of pathogenic viruses, bacteria and parasites using molecular methods, which are fast and highly sensitive to detect microbial pathogens in various specimens. MBG is an ISO 17025 accredited facility and benefits from an isolated containment level 3 facility for processing highly (level 3) contagious pathogens.


All Bacteria Virus Fungi Parasite


Accredited *
Assay Code See Below
Description African horse sickness (AHS) virus is a double stranded RNA virus of the family Reoviridae and causes African horse sickness in horses and related species such as mules, donkeys, and zebras. Horses are most severely affected by the disease. AHS is endemic in the central tropical regions of Africa and has occasionally extended to Egypt, the Middle East and the southern Arabian Peninsula.

The disease spreads mainly by the bite of insects such as midges (Culicoides) but direct horse to horse transmission does not occur. The virus can only survive through continuous cycles of transmission between its hosts-horses and insects. It does not survive in the environment outside of the host. Clinical signs are typically seen five to seven days after infection in the form of fever, redness of the inside surface of the eyelids. The disease progresses to one of the following forms: pulmonary form, cardiac form or mixed form.

There are nine distinct serotypes of AHSV (AHSV-1 to AHSV-9) . Any of the strains can cause disease with severity ranging from a mild fever to sudden death. The serotypes can be distinguished in serum neutralisation tests by the specificity of their reactions with neutralising antibodies. Molecular methods for determination of AHSV serotypes have been developed based on a set of nine individual ‘typing’ assays to detect and identify Seg-2 of each AHSV serotype.

Pathogens Tested
  • APA-001 : African horse sickness virus*
  • APA-182 : African horse sickness virus Serotyping

* Accredited Tests.

Method Real -Time RT-PCR
Sample Type
Accredited : EDTA Blood, Tissue (Spleen, Lung, Lymph nodes) , Culture.
Transport Condition Samples should be transported at 4°C and delivered within 24h of collection
Turn Around Time (TAT) Normal Turnaround time for pathogen Identification is within 3 working days.
Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.

Links

Validated
Assay Code APA-002
Description The adenoviruses are a group of non-enveloped, icosahedral DNA viruses. Adenovirus infections are ubiquitous in commercially farmed birds, and probably in all avian species.

The avian adenoviruses can be divided into three groups. Group I, or conventional adenoviruses, share a common group antigen. Group II adenoviruses include the viruses of turkey haemorrhagic enteritis (THE), marble spleen disease (MSD) and group II splenomegaly of chickens. These viruses also share a common antigen. Group III viruses, the egg drop syndrome (EDS) viruses, are widely distributed in waterfowl but can easily infect chickens, resulting in the production of abnormal eggshells. While many infections are subclinical and appear to be of little economic or welfare importance, significant outbreaks of disease associated with adenovirus do occur.

Method PCR and gel electrophoresis
Sample Type
Stool, Tissue (trachea, bursa, nasal mucosa, pharynx, lungs, kidney), Culture, Swab/Secretion (Rectal)
Transport Condition Samples should be transported at 4°C and delivered within 24h of collection.
Turn Around Time (TAT) Normal Turnaround time for pathogen Identification is within 3 working days.
Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.

Links