Stem cell research and Cytogenetics have been utilized for years to help understand cell and tissue regeneration as well as how chromosomal abnormalities and genetic diseases occur. We in MBG provide dependable, high-quality results with fast turnaround time by microscopic visualizing of the chromosomes allowing our clients to identify the presence of any chromosomal anomalies such as deletions, inversions, and duplications.
Cytogenetics provides the basis for identification, analysis, and understanding of chromosome configuration and behavior within a species that may be of economic and/or scientific interest.
Chromosome analysis provides a "bird’s eye view" of an individual's genetic information. Missing, extra, or rearranged chromosome material may be responsible for diverse problems such as birth defects, mental retardation, autism, infertility, and repeated miscarriage in humans. Chromosome karyotypes are prepared from lymphocytes of peripheral blood or tissue fibroblasts which have been isolated and cultured under proper laboratory conditions. These cells are then characterized by using a special imaging microscope. Analysis of numerical, by counting to ensure that the cells evaluated have the correct number of chromosomes, and structural, to ensure that there are no large pieces of material that are missing (deleted), extra (duplicated), or any rearranged.
It is important to realize that standard chromosome analysis may not be able to detect tiny deletions or duplications of genetic material, and will not be able to detect single-gene mutations.
FISH is considered a cytogenetic as well as a molecular technique. It depends on the use of a nucleic acid probe that hybridizes to the entire chromosome or a single unique DNA sequence. FISH helps in the detection of gains or losses in DNA as well as localization of any DNA fragment. FISH has been used as an important diagnostic tool in the identification of chromosomal abnormalities like duplications, deletions, and translocations. It can also be used for the mapping of genes on the chromosomes. The process can be compared to looking for a needle in a haystack, with the needle being the DNA sequence of interest and the haystack being a set of chromosomes. Fluorescent labeled probes (a specific sequence of nucleotides that is complementary to the DNA sequence of interest) are used as a magnet to pull out the DNA sequence from the entire spread of the chromosome.
Assays Available
HOT-081 : Chromosomal Analysis (Karyotyping)
HOT-082 : Fluorescent In Situ Hybridization (FISH)
Method
Karyotyping / Fluorescent in situ hybridization (FISH).
Sample Type
Fresh heparin blood.
Turn Around Time (TAT)
Depends on the type of sample. Kindly contact MBG Lab for further details.
Stem cell research and Cytogenetics have been utilized for years to help understand cell and tissue regeneration as well as how chromosomal abnormalities and genetic diseases occur. We in MBG provide dependable, high-quality results with fast turnaround time by microscopic visualizing of the chromosomes allowing our clients to identify the presence of any chromosomal anomalies such as deletions, inversions, and duplications.
Cytogenetics provides the basis for identification, analysis, and understanding of chromosome configuration and behavior within a species that may be of economic and/or scientific interest.
Chromosome analysis provides a "bird’s eye view" of an individual's genetic information. Missing, extra, or rearranged chromosome material may be responsible for diverse problems such as birth defects, mental retardation, autism, infertility, and repeated miscarriage in humans. Chromosome karyotypes are prepared from lymphocytes of peripheral blood or tissue fibroblasts which have been isolated and cultured under proper laboratory conditions. These cells are then characterized by using a special imaging microscope. Analysis of numerical, by counting to ensure that the cells evaluated have the correct number of chromosomes, and structural, to ensure that there are no large pieces of material that are missing (deleted), extra (duplicated), or any rearranged.
It is important to realize that standard chromosome analysis may not be able to detect tiny deletions or duplications of genetic material, and will not be able to detect single-gene mutations.
FISH is considered a cytogenetic as well as a molecular technique. It depends on the use of a nucleic acid probe that hybridizes to the entire chromosome or a single unique DNA sequence. FISH helps in the detection of gains or losses in DNA as well as localization of any DNA fragment. FISH has been used as an important diagnostic tool in the identification of chromosomal abnormalities like duplications, deletions, and translocations. It can also be used for the mapping of genes on the chromosomes. The process can be compared to looking for a needle in a haystack, with the needle being the DNA sequence of interest and the haystack being a set of chromosomes. Fluorescent labeled probes (a specific sequence of nucleotides that is complementary to the DNA sequence of interest) are used as a magnet to pull out the DNA sequence from the entire spread of the chromosome.
Assays Available
HOT-081 : Chromosomal Analysis (Karyotyping)
HOT-082 : Fluorescent In Situ Hybridization (FISH)
Method
Karyotyping / Fluorescent in situ hybridization (FISH).
Sample Type
Fresh heparin blood.
Turn Around Time (TAT)
Depends on the type of sample. Kindly contact MBG Lab for further details.
