Molecular based methods have advanced the centre testing to faster and better diagnostics. PCR methods, microarray and next generation sequencing have provided speed and
high level of accuracy. Molecular biology based methods are sensitive and quick to detect microbial pathogens in various clinical specimens.
We offer molecular detection of pathogenic viruses, bacteria and parasites from clinical specimens. MBG is ISO 15189 accredited and benefits from a stand-alone
containment Level 3 facility where samples for highly contagious pathogen are received and processed.
Nipah virus (NiV) is a member of the family Paramyxoviridae, genus Henipavirus. Transmission of Nipah virus to humans may occur after direct contact with infected bats, infected pigs, or from other NiV infected people. Infection with Nipah virus is associated with encephalitis (inflammation of the brain). After exposure and an incubation period of 5 to 14 days, illness presents with 3-14 days of fever and headache, followed by drowsiness, disorientation and mental confusion. These signs and symptoms can progress to coma within 24-48 hours. Some patients have a respiratory illness during the early part of their infections, and half of the patients showing severe neurological signs showed also pulmonary signs. During the Nipah virus disease outbreak in 1998-99, 265 patients were infected with the virus. About 40% of those patients who entered hospitals with serious nervous disease died from the illness. Long-term sequelae following Nipah virus infection have been noted, including persistent convulsions and personality changes. Latent infections with subsequent reactivation of Nipah virus and death have also been reported months and even years after exposure.
Method
Real-Time RT-PCR.
Sample Type
EDTA Blood, CSF, Swab / Secretion (Respiratory)
Transport Condition
Samples should be transported at 4°C. Urine sample must be frozen after collection and delivered within 24 hours.Specimens must be sent in RNA Preservative media. Contact MBG Lab for specimen tubes containing RNA preservative if required.
Turn Around Time (TAT)
TAT for routine samples is within 3 working days. Samples delivered after 11:00 AM will be processed next working day (unless urgent).
Urgent Samples will be reported within 24-48 hours and will be charged double.
Depending on the requirement of the customer, high-quality DNA or RNA will be extracted from human samples. The yield will depend on the nature of the sample presented. Fresh samples are preferred over samples stored for long periods to obtain better yields.
RNA is extremely sensitive and unstable unless stored under appropriate conditions. After extraction, OD analysis for purity, electrophoresis for integrity, and RT-real time PCR analysis for housekeeping genes can be performed to ensure the quality of RNA.
Pathogens Tested
Nucleic acid Extraction (DNA/ RNA)
Nucleic acid Extraction with quantification (DNA/ RNA)
Nucleic acid Extraction with quality check (DNA/ RNA)
Method
Manual or Automated methods will be used depending on the nature of the sample.
Sample Type
Accredited :
1
Alternatives :
Please contact MBG lab for information
Transport Condition
555
Turn Around Time (TAT)
TAT for routine samples is within 3 working days. Samples delivered before 11:00 AM will begin processing immediately resulting in shorter TAT. Urgent Samples must be delivered before 11:00 AM and will be charged double.
Molecular based methods have advanced the centre testing to faster and better diagnostics. PCR methods, microarray and next generation sequencing have provided speed and
high level of accuracy. Molecular biology based methods are sensitive and quick to detect microbial pathogens in various clinical specimens.
We offer molecular detection of pathogenic viruses, bacteria and parasites from clinical specimens. MBG is ISO 15189 accredited and benefits from a stand-alone
containment Level 3 facility where samples for highly contagious pathogen are received and processed.
Nipah virus (NiV) is a member of the family Paramyxoviridae, genus Henipavirus. Transmission of Nipah virus to humans may occur after direct contact with infected bats, infected pigs, or from other NiV infected people. Infection with Nipah virus is associated with encephalitis (inflammation of the brain). After exposure and an incubation period of 5 to 14 days, illness presents with 3-14 days of fever and headache, followed by drowsiness, disorientation and mental confusion. These signs and symptoms can progress to coma within 24-48 hours. Some patients have a respiratory illness during the early part of their infections, and half of the patients showing severe neurological signs showed also pulmonary signs. During the Nipah virus disease outbreak in 1998-99, 265 patients were infected with the virus. About 40% of those patients who entered hospitals with serious nervous disease died from the illness. Long-term sequelae following Nipah virus infection have been noted, including persistent convulsions and personality changes. Latent infections with subsequent reactivation of Nipah virus and death have also been reported months and even years after exposure.
Method
Real-Time RT-PCR.
Sample Type
EDTA Blood, CSF, Swab / Secretion (Respiratory)
Transport Condition
Samples should be transported at 4°C. Urine sample must be frozen after collection and delivered within 24 hours.Specimens must be sent in RNA Preservative media. Contact MBG Lab for specimen tubes containing RNA preservative if required.
Turn Around Time (TAT)
TAT for routine samples is within 3 working days. Samples delivered after 11:00 AM will be processed next working day (unless urgent).
Urgent Samples will be reported within 24-48 hours and will be charged double.
Depending on the requirement of the customer, high-quality DNA or RNA will be extracted from human samples. The yield will depend on the nature of the sample presented. Fresh samples are preferred over samples stored for long periods to obtain better yields.
RNA is extremely sensitive and unstable unless stored under appropriate conditions. After extraction, OD analysis for purity, electrophoresis for integrity, and RT-real time PCR analysis for housekeeping genes can be performed to ensure the quality of RNA.
Pathogens Tested
Nucleic acid Extraction (DNA/ RNA)
Nucleic acid Extraction with quantification (DNA/ RNA)
Nucleic acid Extraction with quality check (DNA/ RNA)
Method
Manual or Automated methods will be used depending on the nature of the sample.
Sample Type
Accredited :
1
Alternatives :
Please contact MBG lab for information
Transport Condition
555
Turn Around Time (TAT)
TAT for routine samples is within 3 working days. Samples delivered before 11:00 AM will begin processing immediately resulting in shorter TAT. Urgent Samples must be delivered before 11:00 AM and will be charged double.
