Molecular Biology & Genetics Laboratory

Species Specific Services


Services grouped by species of interest.


Avian Camelid Equine Feline Canine


Validated    
Assay Code See Below
Description Burkholderia mallei is a gram negative bipolar aerobic bacterium belonging to the genus Burkholderia. It causes a contagious and fatal disease in horses, donkeys, and mules which is called Glanders. According to the location of the initial infection, the disease presents itself in four different forms; nasal, pulmonary, cutaneous and asymptomatic carrier. The nasal and pulmonary forms tend to be more acute while the cutaneous form is a chronic process. Inflammatory nodules and ulcers develop in the nasal passages and give rise to a sticky yellow discharge. Stellate scarring follows upon healing of the ulcers. The formation of nodular abscesses in the lungs is accompanied by progressive debility, coughing and may also be accompanied by diarrhoea. In the cutaneous form (farcy), the lymph vessels are enlarged; nodular abscesses form along their course, which then ulcerate and discharge yellow pus. Nodules are regularly found in the liver and spleen, leading to wasting and death.Control of glanders requires early detection and diagnostic testing of suspected clinical cases, screening of apparently normal equids, and elimination of positive cases.

Burkholderia pseudomallei infects animals and causes the disease melioidosis. B. pseudomallei is an opportunistic pathogen and affects many animal species; infection generally results from grazing on contaminated soil or drinking contaminated water. Infected animals can excrete the organism in saliva, pus, urine, and feces. Severe disease occurs in sheep and goats but cattle, dogs, cats, horses, buffalo, rodents, camels, nonhuman primates, some species of birds, and tropical fish, also get infected. The incubation period for animals is variable ranging from a few days to many years. Some abscesses are carried asymptotically. The signs of melioidosis in animals vary depending on species, but generally include depression, fever, weight loss, respiratory signs (heavy breathing, sneezing), lameness and swelling of the joints, and potentially death. Any animals showing signs of illness should be promptly isolated.

Pathogens Tested
  • APB-026 : Burkholderia mallei.
  • APB-027 : Burkholderia pseudomallei.

Method Real-Time PCR
Sample Type
Swab/Secretion (Respiratory), Tissue ( ulcers, trachea, larynx, lymph nodes, lesions), Semen, Culture.
Transport Condition Samples should be transported at 4°C.
Turn Around Time (TAT) Normal Turnaround time for pathogen Identification is within 5 working days.
Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.

Links




Molecular Biology & Genetics Laboratory

Species Specific Services


Services grouped by species of interest.


Avian Camelid Equine Feline Canine


Validated    
Assay Code See Below
Description Burkholderia mallei is a gram negative bipolar aerobic bacterium belonging to the genus Burkholderia. It causes a contagious and fatal disease in horses, donkeys, and mules which is called Glanders. According to the location of the initial infection, the disease presents itself in four different forms; nasal, pulmonary, cutaneous and asymptomatic carrier. The nasal and pulmonary forms tend to be more acute while the cutaneous form is a chronic process. Inflammatory nodules and ulcers develop in the nasal passages and give rise to a sticky yellow discharge. Stellate scarring follows upon healing of the ulcers. The formation of nodular abscesses in the lungs is accompanied by progressive debility, coughing and may also be accompanied by diarrhoea. In the cutaneous form (farcy), the lymph vessels are enlarged; nodular abscesses form along their course, which then ulcerate and discharge yellow pus. Nodules are regularly found in the liver and spleen, leading to wasting and death.Control of glanders requires early detection and diagnostic testing of suspected clinical cases, screening of apparently normal equids, and elimination of positive cases.

Burkholderia pseudomallei infects animals and causes the disease melioidosis. B. pseudomallei is an opportunistic pathogen and affects many animal species; infection generally results from grazing on contaminated soil or drinking contaminated water. Infected animals can excrete the organism in saliva, pus, urine, and feces. Severe disease occurs in sheep and goats but cattle, dogs, cats, horses, buffalo, rodents, camels, nonhuman primates, some species of birds, and tropical fish, also get infected. The incubation period for animals is variable ranging from a few days to many years. Some abscesses are carried asymptotically. The signs of melioidosis in animals vary depending on species, but generally include depression, fever, weight loss, respiratory signs (heavy breathing, sneezing), lameness and swelling of the joints, and potentially death. Any animals showing signs of illness should be promptly isolated.

Pathogens Tested
  • APB-026 : Burkholderia mallei.
  • APB-027 : Burkholderia pseudomallei.

Method Real-Time PCR
Sample Type
Swab/Secretion (Respiratory), Tissue ( ulcers, trachea, larynx, lymph nodes, lesions), Semen, Culture.
Transport Condition Samples should be transported at 4°C.
Turn Around Time (TAT) Normal Turnaround time for pathogen Identification is within 5 working days.
Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.

Links