Molecular Biology & Genetics Laboratory

Genomics


Animal genomics play a critical role in animal health and disease. The sequencing of many animal genomes has helped to develop powerful tools with which to investigate inherited traits and diseases. MBG Lab animal genomics services deliver innovative, affordable molecular testing for furthers understanding of which animals are at risk for particular diseases and to differentiate between different breeds of animals. The ongoing discoveries in this field can also lead to the development of diagnostic tests and potential therapies for treatment, resulting in better health outcomes and early disease intervention.

Validated
Assay Code XXXXXX
Description The sequencing of many animal genomes facilitates the investigation of inherited traits and diseases. These resources empower scientists with the ability to study novel genetic mutations and enable better understanding of genetic defects and disease susceptibilities across species. MBG laboratory is continuously breaking new ground and deepening the understanding of the animal world through advancements in this field.

Method Next Generation Sequencing (NGS) inclusive of Bioinformatics analysis and Interpretation.
Sample Type
EDTA blood: >2.5ml of blood in EDTA tube.
Fresh Tissue: >50mg tissue in saline dampened gauze.
Transport Condition EDTA blood: Transport under cold conditions.
Fresh Tissue: to be received within 2hours of operation; or snap frozen in liquid nitrogen within 2hours of operation and stored below -70°C. Transport on dry ice.
Note Please refer to the service list for further information regarding requirements.

Links

Validated
Assay Code AGT-161
Description Microbial whole genome sequencing is significant tool for mapping genomes of novel microorganisms, re-sequencing known organisms, or comparing the genomes of across multiple samples. Sequencing the whole genome of bacteria, virus, and other microorganisms is vital to generate reference genomes, identify microbes, and for other comparative genomic studies. Next-generation sequencing (NGS) allows researchers to sequence many organisms in one run. NGS can identify the genome rearrangements and the low frequency variants that maybe missed or are difficult to identify using other methods.

Method Next Generation Sequencing (NGS) inclusive of Bioinformatics analysis and Interpretation.
Sample Type
Isolates.

Links

Validated
Assay Code AGT-162
Description The16S ribosomal RNA (rRNA) sequencing is common amplicon sequencing method used to identify and compare bacteria present in unknown sample. The 16S rRNA gene sequencing is well-established method for comparing sample phylogeny and taxonomy from complex microbiomes or environments that are difficult to study. The next generation sequencing of the 16s rRNA allows culture free testing from complex microbial samples to detect and identify several pathogens in parallel. The method produces a higher resolution in less time to results for bacterial identification compared to other identification methods

Method Next Generation Sequencing (NGS) inclusive of Bioinformatics analysis and Interpretation.
Sample Type
Numerous (Contact MBG)

Links

Validated
Assay Code AGT-159
Description Analysis of the whole mitochondrial Genome. This can be used for evolution and ancestry studies. This assay can also be used for breed identification and mitochondrial related disorders.

Method Next Generation Sequencing (NGS) inclusive of Bioinformatics analysis and Interpretation.
Sample Type
EDTA blood, Tissue, Biopsy.
Transport Condition Samples should be transported at 4°C

Links

Validated
Assay Code XXXX
Description MBG Lab houses the state-of-the art, high resolution Gene Titan Multi-Channel (MC). The instrument is an automated High-throughput solution for monitoring gene expression and genome-wide SNP genotyping. The Instrument automates array processing from target hybridization to data generation by combining a hybridization oven, fluidics processing, and state-of-the art imaging device into a single bench-top instrument for use in genome-wide association studies (GWAS), replication studies, candidate-gene association, and targeted genotyping for animal genomics. GeneTitan MC Instrument delivers the highest throughput and laboratory productivity by minimizing user intervention and allowing unattended, overnight processing of large numbers of samples in parallel. This unique degree of hands-free automation means that the data is consistently reproducible.

Panels Available Various arrays are available for different animal species including :
  • Equine
  • Bovine
  • Others species
    Including but not limited to :
    Chicken, Canine, Feline.

Method Microarray.
Sample Type
EDTA Blood.
Transport Condition EDTA blood: >2.5ml of blood in EDTA tube. Transport under cold conditions.

Please, contact MBG Lab for more information

Validated
Assay Code AOT-062
Description MBG Lab provides DNA sequencing services to clients at public and private institutions. Sanger sequencing platforms are available, providing inexpensive sequencing of isolated fragments. We can also work with researchers to carry out services that may not be listed here. We can generate custom charges based on our estimates of what the work will cost. If you don't see something you want us to do, please inquire whether we can carry out this service.

Method Sanger Sequencing
Sample Type
Kindly contacts the MBG Lab for sample type recommendations.

Links




Molecular Biology & Genetics Laboratory

Genomics


Animal genomics play a critical role in animal health and disease. The sequencing of many animal genomes has helped to develop powerful tools with which to investigate inherited traits and diseases. MBG Lab animal genomics services deliver innovative, affordable molecular testing for furthers understanding of which animals are at risk for particular diseases and to differentiate between different breeds of animals. The ongoing discoveries in this field can also lead to the development of diagnostic tests and potential therapies for treatment, resulting in better health outcomes and early disease intervention.

Validated
Assay Code XXXXXX
Description The sequencing of many animal genomes facilitates the investigation of inherited traits and diseases. These resources empower scientists with the ability to study novel genetic mutations and enable better understanding of genetic defects and disease susceptibilities across species. MBG laboratory is continuously breaking new ground and deepening the understanding of the animal world through advancements in this field.

Method Next Generation Sequencing (NGS) inclusive of Bioinformatics analysis and Interpretation.
Sample Type
EDTA blood: >2.5ml of blood in EDTA tube.
Fresh Tissue: >50mg tissue in saline dampened gauze.
Transport Condition EDTA blood: Transport under cold conditions.
Fresh Tissue: to be received within 2hours of operation; or snap frozen in liquid nitrogen within 2hours of operation and stored below -70°C. Transport on dry ice.
Note Please refer to the service list for further information regarding requirements.

Links

Validated
Assay Code AGT-161
Description Microbial whole genome sequencing is significant tool for mapping genomes of novel microorganisms, re-sequencing known organisms, or comparing the genomes of across multiple samples. Sequencing the whole genome of bacteria, virus, and other microorganisms is vital to generate reference genomes, identify microbes, and for other comparative genomic studies. Next-generation sequencing (NGS) allows researchers to sequence many organisms in one run. NGS can identify the genome rearrangements and the low frequency variants that maybe missed or are difficult to identify using other methods.

Method Next Generation Sequencing (NGS) inclusive of Bioinformatics analysis and Interpretation.
Sample Type
Isolates.

Links

Validated
Assay Code AGT-162
Description The16S ribosomal RNA (rRNA) sequencing is common amplicon sequencing method used to identify and compare bacteria present in unknown sample. The 16S rRNA gene sequencing is well-established method for comparing sample phylogeny and taxonomy from complex microbiomes or environments that are difficult to study. The next generation sequencing of the 16s rRNA allows culture free testing from complex microbial samples to detect and identify several pathogens in parallel. The method produces a higher resolution in less time to results for bacterial identification compared to other identification methods

Method Next Generation Sequencing (NGS) inclusive of Bioinformatics analysis and Interpretation.
Sample Type
Numerous (Contact MBG)

Links

Validated
Assay Code AGT-159
Description Analysis of the whole mitochondrial Genome. This can be used for evolution and ancestry studies. This assay can also be used for breed identification and mitochondrial related disorders.

Method Next Generation Sequencing (NGS) inclusive of Bioinformatics analysis and Interpretation.
Sample Type
EDTA blood, Tissue, Biopsy.
Transport Condition Samples should be transported at 4°C

Links

Validated
Assay Code XXXX
Description MBG Lab houses the state-of-the art, high resolution Gene Titan Multi-Channel (MC). The instrument is an automated High-throughput solution for monitoring gene expression and genome-wide SNP genotyping. The Instrument automates array processing from target hybridization to data generation by combining a hybridization oven, fluidics processing, and state-of-the art imaging device into a single bench-top instrument for use in genome-wide association studies (GWAS), replication studies, candidate-gene association, and targeted genotyping for animal genomics. GeneTitan MC Instrument delivers the highest throughput and laboratory productivity by minimizing user intervention and allowing unattended, overnight processing of large numbers of samples in parallel. This unique degree of hands-free automation means that the data is consistently reproducible.

Panels Available Various arrays are available for different animal species including :
  • Equine
  • Bovine
  • Others species
    Including but not limited to :
    Chicken, Canine, Feline.

Method Microarray.
Sample Type
EDTA Blood.
Transport Condition EDTA blood: >2.5ml of blood in EDTA tube. Transport under cold conditions.

Please, contact MBG Lab for more information

Validated
Assay Code AOT-062
Description MBG Lab provides DNA sequencing services to clients at public and private institutions. Sanger sequencing platforms are available, providing inexpensive sequencing of isolated fragments. We can also work with researchers to carry out services that may not be listed here. We can generate custom charges based on our estimates of what the work will cost. If you don't see something you want us to do, please inquire whether we can carry out this service.

Method Sanger Sequencing
Sample Type
Kindly contacts the MBG Lab for sample type recommendations.

Links