Molecular Biology & Genetics Laboratory

Genomics


The advancement of sequencing technologies, computational science and database contributed to the unexpected leap in knowledge of the human genome that plays a vital role in predicting, preventing and diagnosing of diseases. MBG Lab houses two next generation sequencers enabling the laboratory to expand its repertoire of services into areas such as meta-genomics, whole genome and exome sequencing along with high throughput targeted panel sequencing. MBG Lab benefits from high-performance computing (HPC) infrastructure and validated in-house pipelines to process and assist in interpreting the immense genomic sequence data, rapidly and accurately.
For any of the below services kindly contact MBG Lab in advance.

Validated
Assay Code HGG-125
Description Sequencing of the whole human genome. Genomic information has been instrumental in identifying inherited and metabolic disorders and characterizing the mutations that drive cancer progression.

Method Next Generation Sequencing (NGS) inclusive of Bioinformatics analysis and Clinical Interpretation.
Sample Type
EDTA Blood, Fresh Tissue, Buccal Swab.
Transport Condition EDTA blood: >2.5ml of blood in EDTA tube. Transport under cold conditions.

Fresh Tissue: >50mg tissue in saline dampened gauze to be received within 2hours of operation; or snap frozen in liquid nitrogen within 2hours of operation and stored below -70°C. Transport on dry ice.

Buccal Swab: Do not eat / drink for >1hour before collection. Rub swab inside the mouth with firm pressure for 1min. Place in the collection tube, seal and store between -20°C to 25°C. Transport within 48 hours.

Note Please refer to the service list below for the price and minimal sample number requirements.
A consent form must be completed in full or the sample will be rejected.

Links

Validated
Assay Code HGE-126
Description Next Generation Sequencing of the human exome. The exome consists of all the genome’s exons, which are the coding portions of genes. This technique provides high depth sequencing of the coding regions (exons) in comparison to Whole Genome Sequencing.

Method Next Generation Sequencing (NGS) inclusive of Bioinformatics analysis and Clinical Interpretation.
Sample Type
EDTA Blood, Fresh Tissue, Buccal Swab.
Transport Condition EDTA blood: >2.5ml of blood in EDTA tube. Transport under cold conditions.

Fresh Tissue: >50mg tissue in saline dampened gauze to be received within 2hours of operation; or snap frozen in liquid nitrogen within 2hours of operation and stored below -70°C. Transport on dry ice.

Buccal Swab: Do not eat / drink for >1hour before collection. Rub swab inside the mouth with firm pressure for 1min. Place in the collection tube, seal and store between -20°C to 25°C. Transport within 48 hours.

Note Please refer to the service list below for the price and minimal sample number requirements.
A consent form must be completed in full or the sample will be rejected.

Links

Validated
Assay Code See Below
Description Targeted next generation sequencing allows you to sequence specific areas of the genome for in-depth analyses more rapidly and cost-effectively than whole genome or whole exome sequencing. Targeted sequencing uses deep sequencing to detect known and novel variants within your region of interest.

MBG targeted panels have a select number of specific genes, or coding regions within genes that are known to harbor mutations that contribute to pathogenesis of disease, and include clinically actionable genes of interest.  We have a range of panels available depending on your disease of interest. Contact us for more details.

Panels Available
  • HGT-186 : Targeted Clinical Exome Sequencing (Includes >4800 clinically relevant genes)
  • HGT-128 : Comprehensive Autism Panel (61 genes)
  • HGT-129 : Comprehensive Cardio-vascular Panel (29 genes)
  • HGT-130 : Comprehensive Cardio-myopathy Panel (67 genes)
  • HGT-131 : Sudden Cardiac Arrest Panel (13 genes)
  • HGT-133 : Diabetes MODY Panel (12 genes)
  • HGT-134 : Comprehensive Epilepsy Panel (64 genes)
  • HGT-135 : Gastro-Intestinal Panel (21 genes)
  • HGT-138 : Congenital Muscular Dystrophy Panel (30 genes)
  • HGT-139 : LGMD Muscular Dystrophy Panel (34 genes)
  • HGT-140 : Neuro-Muscular Panel (54 genes)
  • HGT-141 : Cystic Fibrosis &Pulmonary Disease Panel (51 genes)
  • HGT-143 : Deafness Panel (23 genes)
  • HGT-144 : Dysmorphic - Dysplasia Panel (62 genes)
  • HGT-145 : Endocrine Disorder Panel (5 genes)
  • HGT-146 : Metabolic Disorder Panel (40 genes)
  • HGT-147 : Neurological Disorder Panel (110 genes)
  • HGT-148 : Pelvic Inflammatory Disease Panel (29 genes)
  • HGT-149 : Renal Disease Panel (21 genes)
  • HGT-151 : Warfarin sensitivity Panel (2 genes)
  • HGT-150 : Visual Disorder Panel (80 genes)

Method Next Generation Sequencing (NGS) inclusive of Bioinformatics analysis and Clinical Interpretation.
Sample Type
EDTA Blood, Fresh Tissue, Buccal Swab.
Transport Condition EDTA blood: >2.5ml of blood in EDTA tube. Transport under cold conditions.

Fresh Tissue: >50mg tissue in saline dampened gauze to be received within 2hours of operation; or snap frozen in liquid nitrogen within 2hours of operation and stored below -70°C. Transport on dry ice.

Buccal Swab: Do not eat / drink for >1hour before collection. Rub swab inside the mouth with firm pressure for 1min. Place in the collection tube, seal and store between -20°C to 25°C. Transport within 48 hours.

Note Please refer to the service list below for the price and minimal sample number requirements.
A consent form must be completed in full or the sample will be rejected.

Links

Validated
Assay Code See Below
Description Targeted next generation sequencing allows you to sequence specific areas of the genome for in-depth analyses more rapidly and cost-effectively than whole genome or whole exome sequencing. Targeted sequencing uses deep sequencing to detect known and novel variants within your region of interest.

MBG targeted panels have a select number of specific genes, or coding regions within genes that are known to harbor mutations that contribute to pathogenesis of disease, and include clinically actionable genes of interest.  We have a range of panels available depending on your disease of interest. Contact us for more details.

Panels Available
  • HGC-152 : Inherited Breast Cancer Panel (6 genes)
  • HGC-153 : Comprehensive Inherited Cancer Panel (56 genes)
  • HGC-154 : Somatic Cancer Panel (15 genes)

Method Next Generation Sequencing (NGS) inclusive of Bioinformatics analysis and Clinical Interpretation.
Sample Type
EDTA Blood, Fresh Tissue, Buccal Swab.
Transport Condition EDTA blood: >2.5ml of blood in EDTA tube. Transport under cold conditions.

Fresh Tissue: >50mg tissue in saline dampened gauze to be received within 2hours of operation; or snap frozen in liquid nitrogen within 2hours of operation and stored below -70°C. Transport on dry ice.

Buccal Swab: Do not eat / drink for >1hour before collection. Rub swab inside the mouth with firm pressure for 1min. Place in the collection tube, seal and store between -20°C to 25°C. Transport within 48 hours.

Note Please refer to the service list below for the price and minimal sample number requirements.
A consent form must be completed in full or the sample will be rejected.

Links

Validated
Assay Code See Below
Description Human metagenomics study’s different elements of the human microbiome. The technology identifies a community of microorganisms present in biological sample, without the necessity of obtaining pure cultures. In MBG we utilize the next generation sequencing to facilitate the identification of large number microorganisms in the specimen.

Panels Available
  • HGT-161 : Microbial Whole Genome Analysis
  • HGT-162 : Microbial 16S Genome Analysis

Sample Type
Numerous (Contact MBG).
Note Please refer to the service list below for the price and minimal sample number requirements.
A consent form must be completed in full or the sample will be rejected.

Links

Validated
Assay Code See Below
Description Whole mitochondrial genome analysis using next generation sequencing enables us to diagnose and study the different mitochondrial metabolic disorders. In addition to this mitochondrial NGS analysis hugely contributes in the evolutionary and ancestry studies.

Panels Available
  • HGT-136 : Mitochondrial Disorder Panel (Nuclear genes only ; 35 genes)
  • HGT-137 : Mitochondrial Whole Genome Analysis (14 genes)
  • HGT-159 : Ancestral Studies

Method Next Generation Sequencing (NGS) inclusive of Bioinformatics analysis and Clinical Interpretation.
Sample Type
Blood, Tissue, Buccal Swab, Urine.
Transport Condition EDTA blood: >2.5ml of blood in EDTA tube. Transport under cold conditions.

Fresh Tissue: >50mg tissue in saline dampened gauze to be received within 2hours of operation; or snap frozen in liquid nitrogen within 2hours of operation and stored below -70°C. Transport on dry ice.

Buccal Swab: Do not eat / drink for >1hour before collection. Rub swab inside the mouth with firm pressure for 1min. Place in the collection tube, seal and store between -20°C to 25°C. Transport within 48 hours.

Note Please refer to the service list below for the price and minimal sample number requirements.
A consent form must be completed in full or the sample will be rejected.

Links

Validated
Assay Code HGA-158
Description MBG Lab houses the state-of-the art, high resolution Gene Titan Multi-Channel (MC). The instrument is an automated High-throughput solution for monitoring gene expression and genome-wide SNP genotyping. The Instrument automates array processing from target hybridization to data generation by combining a hybridization oven, fluidics processing, and state-of-the art imaging device into a single bench-top instrument for use in genome-wide association studies (GWAS), replication studies, candidate-gene association, and targeted genotyping for human disease research. GeneTitan MC Instrument delivers the highest throughput and laboratory productivity by minimizing user intervention and allowing unattended, overnight processing of large numbers of samples in parallel. This unique degree of hands-free automation means that the data is consistently reproducible.

Panels AvailableVarious human SNP arrays including loss-of-function variants, Pharmacogenomic markers, Inflammation and HLA markers, Copy number variants and other markers associated with human disease.
Please contact MBG Lab for full details.

Method Microarray
Sample Type
EDTA Blood.
Transport Condition EDTA blood: >2.5ml of blood in EDTA tube. Transport under cold conditions.
Note Please refer to the service list below for the price and minimal sample number requirements.
A consent form must be completed in full or the sample will be rejected.

Links

Validated
Assay Code HOT-083
Description MBG Lab provides DNA sequencing services to clients at public and private institutions. Sanger sequencing platforms are available, providing inexpensive sequencing of isolated fragments. We can also work with researchers to carry out services that may not be listed here. We can generate custom charges based on our estimates of what the work will cost. If you don't see something you want us to do, please inquire whether we can carry out this service.

Method Sangers Sequencing
Sample Type
Kindly contacts the MBG Lab for sample type recommendations.
Transport Condition Depends on the type of sample. Kindly contact MBG Lab for further details.

Links




Molecular Biology & Genetics Laboratory

Genomics


The advancement of sequencing technologies, computational science and database contributed to the unexpected leap in knowledge of the human genome that plays a vital role in predicting, preventing and diagnosing of diseases. MBG Lab houses two next generation sequencers enabling the laboratory to expand its repertoire of services into areas such as meta-genomics, whole genome and exome sequencing along with high throughput targeted panel sequencing. MBG Lab benefits from high-performance computing (HPC) infrastructure and validated in-house pipelines to process and assist in interpreting the immense genomic sequence data, rapidly and accurately.
For any of the below services kindly contact MBG Lab in advance.

Validated
Assay Code HGG-125
Description Sequencing of the whole human genome. Genomic information has been instrumental in identifying inherited and metabolic disorders and characterizing the mutations that drive cancer progression.

Method Next Generation Sequencing (NGS) inclusive of Bioinformatics analysis and Clinical Interpretation.
Sample Type
EDTA Blood, Fresh Tissue, Buccal Swab.
Transport Condition EDTA blood: >2.5ml of blood in EDTA tube. Transport under cold conditions.

Fresh Tissue: >50mg tissue in saline dampened gauze to be received within 2hours of operation; or snap frozen in liquid nitrogen within 2hours of operation and stored below -70°C. Transport on dry ice.

Buccal Swab: Do not eat / drink for >1hour before collection. Rub swab inside the mouth with firm pressure for 1min. Place in the collection tube, seal and store between -20°C to 25°C. Transport within 48 hours.

Note Please refer to the service list below for the price and minimal sample number requirements.
A consent form must be completed in full or the sample will be rejected.

Links

Validated
Assay Code HGE-126
Description Next Generation Sequencing of the human exome. The exome consists of all the genome’s exons, which are the coding portions of genes. This technique provides high depth sequencing of the coding regions (exons) in comparison to Whole Genome Sequencing.

Method Next Generation Sequencing (NGS) inclusive of Bioinformatics analysis and Clinical Interpretation.
Sample Type
EDTA Blood, Fresh Tissue, Buccal Swab.
Transport Condition EDTA blood: >2.5ml of blood in EDTA tube. Transport under cold conditions.

Fresh Tissue: >50mg tissue in saline dampened gauze to be received within 2hours of operation; or snap frozen in liquid nitrogen within 2hours of operation and stored below -70°C. Transport on dry ice.

Buccal Swab: Do not eat / drink for >1hour before collection. Rub swab inside the mouth with firm pressure for 1min. Place in the collection tube, seal and store between -20°C to 25°C. Transport within 48 hours.

Note Please refer to the service list below for the price and minimal sample number requirements.
A consent form must be completed in full or the sample will be rejected.

Links

Validated
Assay Code See Below
Description Targeted next generation sequencing allows you to sequence specific areas of the genome for in-depth analyses more rapidly and cost-effectively than whole genome or whole exome sequencing. Targeted sequencing uses deep sequencing to detect known and novel variants within your region of interest.

MBG targeted panels have a select number of specific genes, or coding regions within genes that are known to harbor mutations that contribute to pathogenesis of disease, and include clinically actionable genes of interest.  We have a range of panels available depending on your disease of interest. Contact us for more details.

Panels Available
  • HGT-186 : Targeted Clinical Exome Sequencing (Includes >4800 clinically relevant genes)
  • HGT-128 : Comprehensive Autism Panel (61 genes)
  • HGT-129 : Comprehensive Cardio-vascular Panel (29 genes)
  • HGT-130 : Comprehensive Cardio-myopathy Panel (67 genes)
  • HGT-131 : Sudden Cardiac Arrest Panel (13 genes)
  • HGT-133 : Diabetes MODY Panel (12 genes)
  • HGT-134 : Comprehensive Epilepsy Panel (64 genes)
  • HGT-135 : Gastro-Intestinal Panel (21 genes)
  • HGT-138 : Congenital Muscular Dystrophy Panel (30 genes)
  • HGT-139 : LGMD Muscular Dystrophy Panel (34 genes)
  • HGT-140 : Neuro-Muscular Panel (54 genes)
  • HGT-141 : Cystic Fibrosis &Pulmonary Disease Panel (51 genes)
  • HGT-143 : Deafness Panel (23 genes)
  • HGT-144 : Dysmorphic - Dysplasia Panel (62 genes)
  • HGT-145 : Endocrine Disorder Panel (5 genes)
  • HGT-146 : Metabolic Disorder Panel (40 genes)
  • HGT-147 : Neurological Disorder Panel (110 genes)
  • HGT-148 : Pelvic Inflammatory Disease Panel (29 genes)
  • HGT-149 : Renal Disease Panel (21 genes)
  • HGT-151 : Warfarin sensitivity Panel (2 genes)
  • HGT-150 : Visual Disorder Panel (80 genes)

Method Next Generation Sequencing (NGS) inclusive of Bioinformatics analysis and Clinical Interpretation.
Sample Type
EDTA Blood, Fresh Tissue, Buccal Swab.
Transport Condition EDTA blood: >2.5ml of blood in EDTA tube. Transport under cold conditions.

Fresh Tissue: >50mg tissue in saline dampened gauze to be received within 2hours of operation; or snap frozen in liquid nitrogen within 2hours of operation and stored below -70°C. Transport on dry ice.

Buccal Swab: Do not eat / drink for >1hour before collection. Rub swab inside the mouth with firm pressure for 1min. Place in the collection tube, seal and store between -20°C to 25°C. Transport within 48 hours.

Note Please refer to the service list below for the price and minimal sample number requirements.
A consent form must be completed in full or the sample will be rejected.

Links

Validated
Assay Code See Below
Description Targeted next generation sequencing allows you to sequence specific areas of the genome for in-depth analyses more rapidly and cost-effectively than whole genome or whole exome sequencing. Targeted sequencing uses deep sequencing to detect known and novel variants within your region of interest.

MBG targeted panels have a select number of specific genes, or coding regions within genes that are known to harbor mutations that contribute to pathogenesis of disease, and include clinically actionable genes of interest.  We have a range of panels available depending on your disease of interest. Contact us for more details.

Panels Available
  • HGC-152 : Inherited Breast Cancer Panel (6 genes)
  • HGC-153 : Comprehensive Inherited Cancer Panel (56 genes)
  • HGC-154 : Somatic Cancer Panel (15 genes)

Method Next Generation Sequencing (NGS) inclusive of Bioinformatics analysis and Clinical Interpretation.
Sample Type
EDTA Blood, Fresh Tissue, Buccal Swab.
Transport Condition EDTA blood: >2.5ml of blood in EDTA tube. Transport under cold conditions.

Fresh Tissue: >50mg tissue in saline dampened gauze to be received within 2hours of operation; or snap frozen in liquid nitrogen within 2hours of operation and stored below -70°C. Transport on dry ice.

Buccal Swab: Do not eat / drink for >1hour before collection. Rub swab inside the mouth with firm pressure for 1min. Place in the collection tube, seal and store between -20°C to 25°C. Transport within 48 hours.

Note Please refer to the service list below for the price and minimal sample number requirements.
A consent form must be completed in full or the sample will be rejected.

Links

Validated
Assay Code See Below
Description Human metagenomics study’s different elements of the human microbiome. The technology identifies a community of microorganisms present in biological sample, without the necessity of obtaining pure cultures. In MBG we utilize the next generation sequencing to facilitate the identification of large number microorganisms in the specimen.

Panels Available
  • HGT-161 : Microbial Whole Genome Analysis
  • HGT-162 : Microbial 16S Genome Analysis

Sample Type
Numerous (Contact MBG).
Note Please refer to the service list below for the price and minimal sample number requirements.
A consent form must be completed in full or the sample will be rejected.

Links

Validated
Assay Code See Below
Description Whole mitochondrial genome analysis using next generation sequencing enables us to diagnose and study the different mitochondrial metabolic disorders. In addition to this mitochondrial NGS analysis hugely contributes in the evolutionary and ancestry studies.

Panels Available
  • HGT-136 : Mitochondrial Disorder Panel (Nuclear genes only ; 35 genes)
  • HGT-137 : Mitochondrial Whole Genome Analysis (14 genes)
  • HGT-159 : Ancestral Studies

Method Next Generation Sequencing (NGS) inclusive of Bioinformatics analysis and Clinical Interpretation.
Sample Type
Blood, Tissue, Buccal Swab, Urine.
Transport Condition EDTA blood: >2.5ml of blood in EDTA tube. Transport under cold conditions.

Fresh Tissue: >50mg tissue in saline dampened gauze to be received within 2hours of operation; or snap frozen in liquid nitrogen within 2hours of operation and stored below -70°C. Transport on dry ice.

Buccal Swab: Do not eat / drink for >1hour before collection. Rub swab inside the mouth with firm pressure for 1min. Place in the collection tube, seal and store between -20°C to 25°C. Transport within 48 hours.

Note Please refer to the service list below for the price and minimal sample number requirements.
A consent form must be completed in full or the sample will be rejected.

Links

Validated
Assay Code HGA-158
Description MBG Lab houses the state-of-the art, high resolution Gene Titan Multi-Channel (MC). The instrument is an automated High-throughput solution for monitoring gene expression and genome-wide SNP genotyping. The Instrument automates array processing from target hybridization to data generation by combining a hybridization oven, fluidics processing, and state-of-the art imaging device into a single bench-top instrument for use in genome-wide association studies (GWAS), replication studies, candidate-gene association, and targeted genotyping for human disease research. GeneTitan MC Instrument delivers the highest throughput and laboratory productivity by minimizing user intervention and allowing unattended, overnight processing of large numbers of samples in parallel. This unique degree of hands-free automation means that the data is consistently reproducible.

Panels AvailableVarious human SNP arrays including loss-of-function variants, Pharmacogenomic markers, Inflammation and HLA markers, Copy number variants and other markers associated with human disease.
Please contact MBG Lab for full details.

Method Microarray
Sample Type
EDTA Blood.
Transport Condition EDTA blood: >2.5ml of blood in EDTA tube. Transport under cold conditions.
Note Please refer to the service list below for the price and minimal sample number requirements.
A consent form must be completed in full or the sample will be rejected.

Links

Validated
Assay Code HOT-083
Description MBG Lab provides DNA sequencing services to clients at public and private institutions. Sanger sequencing platforms are available, providing inexpensive sequencing of isolated fragments. We can also work with researchers to carry out services that may not be listed here. We can generate custom charges based on our estimates of what the work will cost. If you don't see something you want us to do, please inquire whether we can carry out this service.

Method Sangers Sequencing
Sample Type
Kindly contacts the MBG Lab for sample type recommendations.
Transport Condition Depends on the type of sample. Kindly contact MBG Lab for further details.

Links