Molecular Biology & Genetics Laboratory

Species Specific Services


Services grouped by species of interest.


Avian Camelid Equine Feline Canine


Validated    
Assay Code See Below
Description Babesia also called Nuttallia, is an apicomplexan parasite that infects red blood cells and is transmitted by ticks. Babesia is a protozoan parasite found to infect vertebrate animals, mostly livestock mammals and birds, but also occasionally humans. To date, more than 100 Babesia species have been scientifically described, which are specific to many species of mammals and birds.
In dogs, a large form of Babesia spp. is represented by Babesia canis and small forms of the disease are represented by Babesia gibsoni, B. conradae, and B. vulpes. Babesia canis includes three subspecies (B. caniscanis, B. canisrossi, and B. canisvogeli), which have been identified based on their cross-immunity, serological testing, vector specificity, and molecular phylogeny. Small Babesia spp. include B. gibsoni which comes from Asia, B. conradae found in dogs in the western United States, and B. microti-like founded in Spain, renamed Theileria annae, and later B. vulpes. Domestic dogs and cats can be infected with these Babesia species Dogs can have subclinical disease (i.e. no clinical signs) or illness that ranges from mild (e.g. lethargy, reduced appetite) to severe (e.g. pallor and weakness related to anemia). Severe disease can result in death. Among the various diagnostic techniques used for detection, Real-time quantitative polymerase chain reaction (real-time PCR) assay is one of the sensitive, reliable and desirable methods for specific detection and quantification of causative agents.
Considering of the geographical distribution of Babesia spp. infections in world, we established a series of one-step real-time PCR assays for detection of specific or genus babesia species as shown below. All assays were optimized at a universal thermal cycling condition, and evaluated under monoplex or multiplex condition for detection of babesia DNA, which will be useful in early diagnosis and consequently addressing the threat of babesia agents in dogs.

Pathogens Tested
  • APB-218 - Babesia species (Excluding Babesia microtigroup)
  • APB-219 - Babesia gibsoni (Asian genotype) (inclusive of Babesia spp.)
  • APB-220 - Canine Babesia (Babesia gibsoni (Asian genotype), Babesia canis canis, Babesia canis rossi, Babesia canis vogeli)
  • APB-221 - Canine Babesia subtyping (Babesia caniscanis, Babesia canisrossi, Babesia canisvogeli, Babesia gibsoni)

Method Real Time RT-PCR/ PCR & sequencing
Sample Type
EDTA Blood, Culture
Transport Condition Sample should be transported at 4°C.
Turn Around Time (TAT) Normal Turnaround time for pathogen Identification is within 5 working days.
Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.

Links




Molecular Biology & Genetics Laboratory

Species Specific Services


Services grouped by species of interest.


Avian Camelid Equine Feline Canine


Validated    
Assay Code See Below
Description Babesia also called Nuttallia, is an apicomplexan parasite that infects red blood cells and is transmitted by ticks. Babesia is a protozoan parasite found to infect vertebrate animals, mostly livestock mammals and birds, but also occasionally humans. To date, more than 100 Babesia species have been scientifically described, which are specific to many species of mammals and birds.
In dogs, a large form of Babesia spp. is represented by Babesia canis and small forms of the disease are represented by Babesia gibsoni, B. conradae, and B. vulpes. Babesia canis includes three subspecies (B. caniscanis, B. canisrossi, and B. canisvogeli), which have been identified based on their cross-immunity, serological testing, vector specificity, and molecular phylogeny. Small Babesia spp. include B. gibsoni which comes from Asia, B. conradae found in dogs in the western United States, and B. microti-like founded in Spain, renamed Theileria annae, and later B. vulpes. Domestic dogs and cats can be infected with these Babesia species Dogs can have subclinical disease (i.e. no clinical signs) or illness that ranges from mild (e.g. lethargy, reduced appetite) to severe (e.g. pallor and weakness related to anemia). Severe disease can result in death. Among the various diagnostic techniques used for detection, Real-time quantitative polymerase chain reaction (real-time PCR) assay is one of the sensitive, reliable and desirable methods for specific detection and quantification of causative agents.
Considering of the geographical distribution of Babesia spp. infections in world, we established a series of one-step real-time PCR assays for detection of specific or genus babesia species as shown below. All assays were optimized at a universal thermal cycling condition, and evaluated under monoplex or multiplex condition for detection of babesia DNA, which will be useful in early diagnosis and consequently addressing the threat of babesia agents in dogs.

Pathogens Tested
  • APB-218 - Babesia species (Excluding Babesia microtigroup)
  • APB-219 - Babesia gibsoni (Asian genotype) (inclusive of Babesia spp.)
  • APB-220 - Canine Babesia (Babesia gibsoni (Asian genotype), Babesia canis canis, Babesia canis rossi, Babesia canis vogeli)
  • APB-221 - Canine Babesia subtyping (Babesia caniscanis, Babesia canisrossi, Babesia canisvogeli, Babesia gibsoni)

Method Real Time RT-PCR/ PCR & sequencing
Sample Type
EDTA Blood, Culture
Transport Condition Sample should be transported at 4°C.
Turn Around Time (TAT) Normal Turnaround time for pathogen Identification is within 5 working days.
Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.

Links