The genotype of the foal is compared with the genotype of suspected Sire or Dam using computer software. The results are issued in the form of a microsatellite verification certificate. The more loci that are amplified, the higher the statistical power of discrimination.
Parentage is based on the exclusion principle. A DNA profile of the animal-based on a number of markers is generated which is compared to the DNA profile of the parents. If the genotype matches with that of the parents and no exclusion can be made, then the parentage is qualified. In the case of a single marker mismatch, several other microsatellites (additional markers) are analysed to verify the parentage for the submitted animal. If still there is no more exclusion, the parentage is qualified with one exclusion which could be due to mutation. [This is rare as microsatellite mutation rates are stable enough for pedigree analysis. Mutation rates are in the order of one mutation in 1,000-1,000,000 generations per given loci]. However, if more markers are excluded the parentage is disqualified.
Parentage exclusions are 100% accurate, whereas parentage qualifications are not. The accuracy of most animal parentage testing is more than 99% if both parents are provided. However, this accuracy drops down to 95% when only one parent is provided for analysis. The accuracy still lowers down if the potential parent(s) are part of a large family of closely related inbred animals. Therefore, to prevent erroneous parentage assignments, it is important to have information for both parents.
Species Tested
Camelid
Equine
Others species
Including but not limited to :
Bovine, Cat, Chicken,
Cheetah, Canine, Caprine,
Dolphin, Falcon, Houbara,
Hyena, Lion, Penguin,
Shark, Sheep, Tiger, Vulture.
Method
STR Analysis
Sample Type
EDTA blood, Hair and tissue. For other type of samples please contact MBG Lab.
Transport Condition
EDTA blood should be transported at 4°C and hair sample at room temperature.
The genotype of the foal is compared with the genotype of suspected Sire or Dam using computer software. The results are issued in the form of a microsatellite verification certificate. The more loci that are amplified, the higher the statistical power of discrimination.
Parentage is based on the exclusion principle. A DNA profile of the animal-based on a number of markers is generated which is compared to the DNA profile of the parents. If the genotype matches with that of the parents and no exclusion can be made, then the parentage is qualified. In the case of a single marker mismatch, several other microsatellites (additional markers) are analysed to verify the parentage for the submitted animal. If still there is no more exclusion, the parentage is qualified with one exclusion which could be due to mutation. [This is rare as microsatellite mutation rates are stable enough for pedigree analysis. Mutation rates are in the order of one mutation in 1,000-1,000,000 generations per given loci]. However, if more markers are excluded the parentage is disqualified.
Parentage exclusions are 100% accurate, whereas parentage qualifications are not. The accuracy of most animal parentage testing is more than 99% if both parents are provided. However, this accuracy drops down to 95% when only one parent is provided for analysis. The accuracy still lowers down if the potential parent(s) are part of a large family of closely related inbred animals. Therefore, to prevent erroneous parentage assignments, it is important to have information for both parents.
Species Tested
Camelid
Equine
Others species
Including but not limited to :
Bovine, Cat, Chicken,
Cheetah, Canine, Caprine,
Dolphin, Falcon, Houbara,
Hyena, Lion, Penguin,
Shark, Sheep, Tiger, Vulture.
Method
STR Analysis
Sample Type
EDTA blood, Hair and tissue. For other type of samples please contact MBG Lab.
Transport Condition
EDTA blood should be transported at 4°C and hair sample at room temperature.